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Dothistroma needle blight (DNB) is a serious needle disease of conifers that primarily affects pine species (Pinus spp.). Dothistroma septosporum is one of the DNB pathogens that has a diverse range of host species excluding Pinus armandii. In 15 inoculated P. armandii seedlings, D. septosporum acervuli were observed in 43 infected needles of ten seedlings with a mean disease severity of 1.11% at 25 weeks after inoculations, demonstrating the potential of D. septosporum to cause symptoms on the needles of P. armandii via artificial inoculation. The disease severity of P. armandii was similar to the positive control, Pinus nigra (median 0.75 for P. armandii to 0.70 for P. nigra), thus, P. armandii acts under artificial conditions as a susceptible host species. 相似文献
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柑橘溃疡病(Xanthomonas citri subsp. citri,Xcc)是严重阻碍柑橘产业发展的世界性检疫病害,防控困难,选育抗病品种为防治溃疡病的唯一有效办法。枸橼C-05是柑橘属独特的抗溃疡病种质,为抗性机理和抗病育种提供了种质基础。为了进一步评价枸橼C-05的抗性,另外收集了13份枸橼种质,对其进行溃疡病的离体和活体抗性评价。叶片形态学观察表明,14份枸橼种质存在较大的形态学差异。采用离体与活体注射接种不同浓度的柑橘溃疡病菌,根据叶片的感病症状、病斑周围黄色晕圈大小等典型溃疡病症状,综合评价枸橼类种质的抗病性。结果表明:枸橼种质之间对溃疡病的抗性存在极大差异,溃疡病的抗性评价综合病情指数显示枸橼C-05、园香橼、矮果香橼、美国枸橼、普通枸橼和印度大果综合病情指数较小,表现为极抗病或抗病,其他枸橼种质表现不同程度的感病性;枸橼种质的抗病或感病性与其叶片形态特征无关。枸橼C-05和5个抗病枸橼种质对柑橘溃疡病具有针对性的抗性,这对进一步研究抗病机理和抗病育种具有重要意义。 相似文献
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针对我国南方地区夏季高温高湿的气候条件,对采用天窗、外遮阳、内喷雾降温措施的试验Venlo温室内温度状况进行了模拟研究。以室外气候参数为边界条件,考虑作物和环境的相互作用,内喷雾系统和室内环境的质热交换以源项的形式加入到控制方程中,采用CFD(computational fluid dynamics)中的稳态方法求解控制方程,模拟Venlo型温室不同调控措施及组合下的温室内温度分布特点,分析各种调控措施的调控效果。模拟结果表明:采用加入源项的方法模拟内喷雾系统和室内空气的质热交换,其模拟值和实测值均方根误差RMSB为0.514 4℃,最大绝对误差为0.75℃,平均相对误差为1.3%,说明所建立的CFD模型有效。3种降温措施下,以外遮阳+自然通风的降温贡献率最大(80.6%),能耗最高的喷雾系统降温贡献率仅为34.8%,较高的环境湿度影响了喷雾系统的降温效率。CFD夏季降温模型的建立为温室作物系统的环境控制策略的制定提供了科学依据。 相似文献
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Breeding wheat and rye for resistance to Fusarium diseases 总被引:6,自引:1,他引:6
T. Miedaner 《Plant Breeding》1997,116(3):201-220
Fusarium culmorum and F. graminearum Groups 1 and 2 cause seedling blight, crown rot, foot rot and head blight in wheat and rye that may affect grain yield and quality for baking and feeding. This review starts with an analysis of Fusarium populations with regard to their genetic variation for aggressiveness, mycotoxin production, and isolate-by-host genotype interaction. To assess resistance in the different host growth stages, quantitative inoculation and disease assessment techniques are necessary. Based on estimated population parameters, breeding strategies are reviewed to improve Fusarium resistance in wheat and rye. Epidemiological and toxicological aspects of Fusarium resistance that are important for resistance breeding are discussed. F. culmorum and F. graminearum display large genetic variation for aggressiveness in isolate collections and in naturally occurring populations. The production of mycotoxins, especially deoxynivalenol and its derivatives, is a common trait in these populations. Significant isolate-by-host genotype interactions were not found across environments in wheat and rye. Artificial infections in the field are indispensable for improving Fusarium crown rot, foot rot and head blight resistance in wheat and rye. For a reliable disease assessment of large populations, disease severity ratings were found to be the most convenient. The differentiation of host resistance is greatly influenced by an array of nongenetic factors (macro-environment, microclimate, host growth stage, host organ) that show significant interactions with host genotype. Selection for environmentally stable resistance has to be performed in several environments under a maximum array of different infection levels. Selection in early growth stages or on one plant organ does not in most cases allow prediction of resistance in adult-plant stages or another plant organ. Significant genetic variation for resistance exists for all Fusarium-incited diseases in breeding populations of wheat and rye. The patho-systems studied displayed a prevalence of additive gene action with no consistent specific combining ability effects and thus rapid progress can be expected from recurrent selection. In wheat, intensive testing of parental genotypes allows good prediction of the mean head blight resistance after crossing. Subsequent selection during selfing generations enables the use of transgression towards resistance. In hybrid breeding of winter rye, the close correlation between foot rot resistance of inbred lines and their GCA effects implies that selection based on the lines per se should be highly effective. This is not valid for F. culmorum head blight of winter rye caused by a greater susceptibility of the inbred lines compared to their crosses. For both foot rot and head blight resistance, a high correlation between the resistance to F. graminearum and F. culmorum was found in wheat and rye. Mycotoxin accumulation occurs to a great extent in naturally and artificially infected plant stands. The correlation between resistance traits and mycotoxin contents are medium and highly dependent on the environment. Further experiments are needed to clarify whether greater resistance will lead to a correlated reduction of the mycotoxin content of the grains under natural infection. 相似文献
58.
Sergio Hernández-Verdugo Ramón G. Guevara-González Rafael F. Rivera-Bustamante Ken Oyama 《Euphytica》2001,122(1):31-36
Plants collected in thirteen wild populations of Capsicum annuum from Northwest Mexico were tested for resistance to the pepper huasteco begomovirus (formerly subgroup III) (PHV) that is
transmitted by the white fly Bemisia tabaci Genadius. Plants were inoculated using both grafting and biolistic methods. Presence of viral DNA was detected by dot blot
hybridization and densitometry. Populations varied in their resistance to PHV. Plants of only two of the populations either
did not develop disease symptoms or showed very light symptoms after inoculation. In some cases, symptoms appeared several
days after inoculation. In plants of these populations viral DNA was detected by dot-blot hybridization assays but they appear
to be a good source of resistance (symptomless) for use in breeding programmes.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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Summary Common blight disease in beans (Phaseolus vulgaris), caused by the bacterium Xanthomonas campestris pv. phaseoli, reduces crop yield and seed quality. Information is needed on the variation of leaves and pods disease reaction to strains of the bacterium after different inoculation methods. Phaseolus vulgaris cultivars Red Kidney Charlevoix, GN Harris, GN 1140, and GN Emerson were inoculated with three different strains of Xanthomonas campestris pv. phaseoli at two inoculum concentrations (108 and 106 bacterial cells/ml) using water soaking, multiple needle, and razor blade inoculation on leaves, and razor blade scratch, dissecting needle, and razor blade cut inoculation on pods. Differential cultivar disease reactions of leaves, pods, or both to the bacterial strains were observed in some cases. Significant interactions among cultivars, inoculation methods, strains, and inoculum concentrations (leaves) were found. A rapid leaf chlorosis developed 6 to 7 days after inoculation. Strains of bacteria did not show specificity in inducing this reaction, but rapid leaf chlorosis was associated with high inoculum concentration and with the water soaking and multiple needle methods. Another experiment was conducted to count the number of living bacterial cells deposited in the leaf tissue after inoculation by different methods. The number of bacteria deposited by water soaking or multiple needle was higher than that deposited by razor blade.Published as Paper No. 8584, Journal Series, Nebraska Agricultural Experiment Station. Research was conducted under Project No. 20–36. 相似文献